This study aimed to determine the enthalpy index in three municipalities of Mato Grosso, to characterize the bioclimatic profile for the poultry industry. In the research, the compensated daily dry bulb temperature and the daily average relative humidity were used. The data corresponding to a historical series of 35 years, between 01/01/1985 and 12/31/2020, recorded in conventional meteorological stations in the municipalities of Canarana, Nova Xavantina, and Santo Antônio de Leverger, available in the Meteorological Database for Teaching and Research of the National Institute of Meteorology (INMET). Then, monthly descriptive statistics of the historical series were determined and bar plot graphs with enthalpy values were developed. These graphs included the lower and upper enthalpy limits, suitable for laying hens and broilers, to qualify the thermal conditions of environmental comfort in the three municipalities of Mato Grosso. It was concluded that the enthalpy index made it possible to characterize the bioclimatic profile of the mentioned municipalities for poultry farming, according to the months of the year. The natural thermal conditions in these municipalities were not favorable to poultry farming, requiring extra investments in the air conditioning of the sheds. The increase in thermal discomfort for broilers was accentuated from the fourth week of the production cycle. The applied methodology presented itself as an adequate tool to assist in the elaboration of projects related to environmental changes in aviaries.